Quantitative model of small molecules uptake after in vitro cell electropermeabilization.

Gene Expression Quantification of Toll like Receptors 2, 4 and Co-molecules in Human Glioblastoma Cell Line (U87-MG): Toward a New In vitro Model of Inflammation

Applications of Small Molecules in Muscle Tissue Engineering

Introduction: Skeletal muscles account for about 40% of the total body weight. Every year, hundreds of people lose at least part of their muscle tissue due to illness, war, and accidents. This can lead to disruption of activities such as breathing, movement, and social life. To this end, various therapeutic strategies such as medication therapy, cell therapy and tissue transplantation have been...

Transport of charged small molecules after electropermeabilization — drift and diffusion

Background Applications of electric-field-induced permeabilization of cells range from cancer therapy to wastewater treatment. A unified understanding of the underlying mechanisms of membrane electropermeabilization, however, has not been achieved. Protocols are empirical, and models are descriptive rather than predictive, which hampers the optimization and expansion of electroporation-based te...

Cell electropermeabilization to small molecules in vitro: control by pulse parameters

The phenomenon of cell membrane electropermeabilization can be described as a dramatic increase in the transmembrane permeability induced by an externally applied electric field. This transient state has important practical applications like the fusion of cells 1 and the introduction of the biologically active substances like drugs 2 and genetic material 3 into cells. Electropermeabilization is...

In-vitro cellular uptake and transport study of 9-nitrocamptothecin PLGA nanoparticles across Caco-2 cell monolayer model

The uptake and transport of 9-nitrocamptothecin (9-NC), a potent anticancer agent, across Caco-2 cell monolayers was studied as a free and PLGA nanoparticle loaded drug. Different sizes (110 to 950 nm) of 9-nitrocamptothecin nanoparticles using poly (lactic-glycolic acid) were prepared by via the nanoprecipitation method. The transport of nanoparticles across the Caco-2 cell monolayer as a func...